ABSTRACT
Aims@#Rice blast, a disease caused by the fungus Magnaporthe grisea is one of the serious diseases of rice in the world. The main objective of this study is to isolate and characterise the proteins extracted from the rice blast fungus, M. grisea 7.6. @*Methodology and results@#Through comparative 2-D analyses of the crude protein extracts obtained from this fungus, we were able to identify 88 protein spots through MALDI-TOF. These proteins were then classified into 8 functional groups through the Pfam and KEGG databases into hypothetical, transferases, energy and carbon metabolism, oxidoreductases, molecular chaperone, hydrolases, structural organisation and kinases. The individual protein’s functions were then identified and their possible role in pathogenesis, virulence and proliferation of M. grisea 7.6 were predicted. @*Conclusion, significance and impact of study@#Through the assays conducted, we were able to identify some proteins and pathways that could be targeted in developing fungicides and used in future mutagenesis studies.
ABSTRACT
WRKY proteins, a big family of transcription factors, are involved in regulation diverse developmental and physiological processes in plants. Here, a novel WRKY gene, OsWRKY52, was isolated from a rice cDNA library. This gene included an open reading frame of 1 719 bp in length, and the deduced polypeptide contained 572 amino acids,sharing 54% identity with a WRKY1 protein from Avena sativa. Expression of OsWRKY52 gene was induced rapidly by Magnaporthe grisea in the incompatible interaction with rice plant. OsWRKY52 protein, expressed prokaryotically bound specifically to W box cis elements derived from the promoter of a rice PR1a. Transcriptional activation assay was performed by a yeast one- hybrid method. Regions of transactivation were identified to be the N-terminal serine- and threonine-rich islands and the C-terminal acidic domain of OsWRKY52. These results suggest that OsWRKY52, as a transcription activator, may be involved in defense responses against Magnaporthe grisea in rice plants.
ABSTRACT
Three hundred and twenty-one bacteria strains were obtained from rice leaves,stem,root tissue and paddy field soil,of which the number of strains which can inhibit mycelium of Magnaporthe grisea growth markedly was fifty-seven through fermentation in 2.0 mL Eppendorf tube,and among these fifty-seven strains,five strains were strongly antagonistic to Magnaporthe grisea.These five strains was identified for their morphologic,physiological and biochemical characteristics,and the results showed that one strain(No.156)was bacillus subtilis,two strains(No.171 and No.177)were Bacillus pumillus and two strains(No.192 and No.279)were Bacillus ploymyxa.